Page 18 - Sensor100 Jan '13
Basic HTML Version
How DoThey DoThat?
In the first of a new feature in
Sensor100
, Bill Jackson of
Base Pair
Technologies
, explores:
Binding Analytics of Aptamers to Small Molecules
determined by MicroscaleThermophoresis
Aptamer development has historically been limited to one-target at
a time.
has addressed this severe limita-
tion by successfully multiplexing the conventional aptamer selection
process. Our platform technology is a patent pending approach to
aptamer discovery that allows us to offer de novo aptamer discov-
ery services at unprecedented speed and throughput. Our expertise
in aptamer and related assay development allows us to support our
customers in a wide range of novel applications.
Our discovery services include validation of aptamer binding by
characterizing the aptamer:target dissociation constant (Kd) before
delivery of aptamer materials to the customer for further testing.
Analytical methods such as Surface Plasmon Resonance (SPR) and
Biolayer Interferometry (BLI) are typically used for larger targets
such as proteins. Recently, however, we have explored and validated
Microscale Thermophoresis (MST) developed by
(München, Germany). In the case of aptamers to
small molecules, MST has no lower molecular weight limit and can
therefore “see” into a range that SPR and BLI cannot. NanoTemper
Technologies GmbH develops, produces and markets innovative, high
quality instruments for bio-medical research based on NanoTem-
per’s unique and proprietary MST technology.
