Page 19 - Sensor100 Jan '13
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Microscale Thermophoresis (MST) is based on a physical principle
used for the first time in bioanalytics. It detects changes in the hy-
dration shell, charge or size of molecules allowing measurement of a
wide range of biomolecular interactions under close-to-native condi-
tions. MST can be performed label-free, immobilization-free, in any
buffer or complex biofluid. NanoTemper’s unique technology is ideal
for basic research applications requiring flexibility in the experimen-
tal scale, as well as for pharmaceutical research applications including
small molecule profiling.
Aptamers are single-stranded DNA or RNA oligonucleotides se-
lected to have unique three dimensional folding structure for bind-
ing to a variety of targets such as proteins, peptides, and even small
molecules with affinity and specificity rivaling that of antibodies.They
are typically selected in vitro by a process commonly referred to as
“SELEX” [1,2] as depicted in Figure 1. Using a proprietary variant
of this process, Base Pair Biotechnologies is developing aptamers to
multiple targets simultaneously.
Schematic of conventional,
single-target DNA aptamer
selection.
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