SiM20 Posters

21 Sensors in Medicine 2020 We developed a new DNA biosensor to achieve single base pair specificity Photo courtesy of http://www.flickr.com/photos/mknowles/ @Channonball r.channon@imperial.ac.uk Ratio 1 st / 2 nd peak Templated EOTR 141-DNA No DNA Non- specific DNA Potential vs Ag/AgCl / V ΔCurrent / µA log peak ratio Log [DNA] The assay sensitivity was tested with differential pulse voltammetry of miRNA-141 (a prostate cancer biomarker) There is a clear signal with 10 nM over the background (flat black line) – note data background subtracted with non-templated reaction (no target) Specificity tested against non- templated reaction (no DNA) and non-specific sequence (miRNA-150) Future work: EOTR sensing of prostate cancer clinical samples and build EOTR into a microfluidic lab-on-a-chip platform.