SiM20 Posters

Sensors in Medicine 2020 24 Diagnosis of bloodstream infections from large sample volume Shantimoy Kar 1 , Xiaoxiang Yan 1 , Nguyen Sy Thau 2 , Andrew Farthing 1 , Melanie Jimenez 1 , Julien Reboud 1 , Ngo Tat Trung 2 , Le Huu Song 2 and Jonathan M Cooper 2 ,* 1 Biomedical Engineering Division, University of Glasgow, G12 8QQ, U.K. 2 Vietnamese-German Center for Medical Research, 108 Military Central Hospital, Hanoi, Vietnam Early stage detection of bacteria bloodstream infections is highly challenging due to the low bacterial counts, requiring lengthy culture steps to increase concentrations above the limits of detection of biosensors. We have developed a point-of-care testing strategy for detecting Escherichia. coli from millilitres of whole blood. The method is based on the treatment of the sample with a mammalian-specific cell lysis buffer 1,2 which enables to enrich E. coli cells on a paper-based origami device. The bacteria are then detected by loop-mediated isothermal amplification (LAMP) integrated in our origami device 3 , reaching a detection limit of 10 3 CFU/mL, a clinically-relevant concentration. Abstract Methods Pre-concentration and lysis Objectives v Developing a point-of-care (POC) testing platform for E. coli detection in blood v Visual interpretable readout assay using isothermal nucleic acid amplification (LAMP) technique DNA purification and transfer to amplification platform Final readout of the assay Step I Step II Step III