SiM20 Posters

25 Sensors in Medicine 2020 Results Primers details 4 F3 GCC ATC TCC TGA TGA CGC 0.2 µ M B3 ATT TAC CGC AGC CAG ACG 0.2 µ M BIP CTG GGG CGA GGT CGT GGT AT TCC GAC AAA CAC CAC GAA TT 1.6 µ M FIP CAT TTT GCA GCT GTA CGC TCG CAG CCC ATC ATG AAT GTT GCT 1.6 µ M Forward Loop CTT TGT AAC AAC CTG TCA TCG ACA 0.8 µ M Backward Loop ATC AAT CTC GAT ATC CAT GAA GGT G 0.8 µ M Ø Mammalian cell lysis buffer offers enables to remove human DN. Ø This strategy was validated for two different E.coli strains (NEB5 alpha and K12) Ø Our device visual readouts on our device offers a rapid detection (~90 minutes directly from 1 mL whole blood) with a sensitivity of 10 3 cells/mL both in hospital settings tested in Vietnam Outlook q Validating the device with patients’ samples and exploring for other pathogen detection q One integrated automated liquid handling platform to minimize manual intervention Sample: 10 3 CFU/mL , NC: negative control, PC: positive control Acknowledgement The work was funded by the Royal Academy of Engineering, through a Frontiers of Engineering in Development FoESF1819\T6\4, as well as EPSRC (EP/P029329/1, EP/R01437X/1) Reservoir i.e. LAMP reaction chambers Finger actuated water pumps Lateral flow strips 0.00E+00 1.50E+05 3.00E+05 4.50E+05 6.00E+05 0 20 40 60 Fluorescence Intensity Heating time (minutes) Negative control 10E6 CFU/mL 10E3 CFU/mL References 1 Trung et al., Scientific Reports (2019), 9, 13663, 2 Trung et.al. , BMC Infectious Diseases, (2016), 16, 235; 3 Reboud et al., PNAS (2019), 116, 4834; 4 Hill et.al. , Journal of Clinical Microbiology, (2008), 46, 2800