SiM20 Posters

35 Sensors in Medicine 2020 Two peaks indicating fluorescence along the lateral flow assay (LFA). One is caused by the OTR at the miR-150-5p test line and the other is the fluorescence at the control line. DETECTION OF microRNA ON PAPER Integrals of fluorescence scan showing difference between oligonucleotide templated (OTR) and non-templated reactions (NTR): ACKNOWLEDGEMENTS: This project is funded by the March of Dimes. CONCLUSION Fluorescence along LFA using Bench-top Scanner • Preliminary results indicate ability to detect multiple miRNAs on a single strip (multiplexed analysis) whilst also incorporating a control line. • A nM LOD was achieved. • The technology proved sensitive enough for amplification-free detection of endogenous concentrations of microRNAs from clinical samples and for efficiently discriminating between women who delivered at term and those who ended up delivering preterm 3 . References: 1. March of Dimes, PMNCH, Save the children, Who. Born Too Soon: The Global action report on preterm Birth. eds cp howson, mV Kinney, Je lawn. World health organization. Geneva, 2012 2. Cook, J.; Bennett, P. R.; Kim, S. H.; Teoh, T. G.; Sykes, L.; Kindinger, L. M.; Garrett, A.; Binkhamis, R.; MacIntyre, D. A.; Terzidou, V. Scientific reports 2019, 9, 5861–5861. 3. Pavagada, S.; Channon, R. B.; Chang, J. Y. H.; Kim, S. H.; MacIntyre, D.; Bennett, P. R.; Terzidou, V.; Ladame, S. Chemical Communications 2019, 55, 12451–12454. miR-150-5p Control line OTR produces a higher fluorescence than NTR (at a concentration of both 10nM and 50nM). The fluorescence increases with increasing concentration of miR-150-5p. A similar fluorescent signal is produced in different conditions (no microRNA, non- complimentary as well as complementary nucleic acids), as the fluorescence is microRNA- independent. miR-150-5p Control line