SiM20 Posters

Sensors in Medicine 2020 36 Abstract: A new luminol probe excels in chemiluminescent bioanalytical strategies S. Rink 1 , A. Duerkop 1 , A. Jacobi von Wangelin 2 , M. Seidel 3 , A. J. Baeumner 1 1 Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg 2 Institute of Inorganic and Applied Chemistry, University of Hamburg 3 Institute of Hydrochemistry, Analytical Chemistry and Water Chemistry, Technical University of Munich Introduction: Luminol is by far the widest used CL compound even though it suffers from limited water solubility affecting its CL quantum yield of approximately 1 %. 1,2 Luminol is oxidized to its light emitting equivalent 3-aminophthalate by H 2 O 2 and typically transition metal cations in aqueous solution. Improving the inherent CL properties of luminol would provide an exceptionally sensitive CL reagent for super sensitive point-of-care applications. New luminol  maintains photophysical characteristics  4-times higher relative quantum yield  CL enhancement in all catalyst systems  up to 3-times enhanced CL signal across the entire CL spectrum Studying the chemiluminescence (CL) of highly water-soluble m- carboxy luminol in bioassays in which H 2 O 2 is either formed or consumed showed consistent CL enhancement towards standard luminol. We studied different catalyst species in microtiter plate and paper-based microfluidic formats and found that our new CL probe excels in bioassays for L-lactate in artificial sweat and diclofenac.