SiM20 Posters

39 Sensors in Medicine 2020 Results (2). Microfluidic device Sample and synthrocytes are mixed, are pipetted in the device (1), and are incubated for 5 min to generate agglutination (2). PBS addition (3) pushes the mixture to the detection zone (4). Here, a paper-based filter separates agglutinated from non-agglutinated particles, forming a colored band that is detectable with the naked eye. This allows performing rapid agglutination tests in under 10 min with little manipulation by the user. Fig. 5 . Front image (a) and side view scheme (b) of the capillarity-driven device produced by laminating low-cost transparency sheets (dark grey) and double-sided adhesive (light grey). Paper components (Whatman G4) appear in blue (detection zone and flow pump). 100 10 1 Neg Lectin (µg/mL) Conclusions Fig. 6 . The colored band formed by agglutinated synthrocytes is proportional to the concentration of agglutinating agent in the sample (a lectin in this case). The device displays currently a sensitivity similar to the original technique (bottom), but requires less reagent, is faster and entails little manipulation by the user.  Synthrocytes are able to bind influenza A(H1N1) and B viruses, agglutinating and producing changes in sedimentation in <10 min that resemble those generated by erythrocytes in 30-60 min.  Synthrocyte-influenza interaction is inhibited by anti-hemagglutinin antibodies, allowing to perform HAI-like assays.  This synthetic reagent can be used with a low-cost laminated device to automatize the process, performing the assay in less than 10 min, requiring less reagent and facilitating the evaluation of the results.  Future work: (i) improvement of synthrocyte sensitivity, selectivity and stability; (ii) automation of HAI-like assays using the microfluidic device and pre-loaded reagents; (iii) validation with clinical samples. Synthrocytes are a potentially mass-produced synthetic reagent, tunable by the customized incorporation of selected bioreceptors, compatible with the use of inexpensive devices for automatization of HA and HAI-like assays. (3) PBS inlet (1) Sample inlet (2) Incubation zone (4) Detection zone (5) Paper pump a) b)