SiM20 Posters

Sensors in Medicine 2020 4 Immunoaffinity Monoliths for Extraction of Multiple Preterm Birth Biomarkers from Blood Serum in 3D Printed Microfluidic Devices Haifa M. Almughamsi , Karyna M. Howell, Samuel R. Parry, Gregory P. Nordin, and Adam T. Woolley Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA ABSTRACT Preterm birth (PTB) is the leading cause of neonatal deaths. Currently, no biomarker-based methods are readily available to determine the risk of PTB prior to onset of labor. We are creating 3D printed microfluidic devices with immunoaffinity monoliths. These monoliths are modified with antibodies to extract and enrich the PTB biomarkers from human blood serum. We have successfully extracted ferritin from human blood serum 1 . This approach was expanded to extract three additional PTB biomarkers. This work shows important progress toward a rapid diagnostic that is capable of purifying all nine PTB biomarkers from human blood serum. Table 1: Panel of nine PTB biomarkers EXPERIMENTAL 3D printing resin components are added and sonicated until fully mixed. 2 • Monomer: 97% poly(ethylene glycol)diacrylate (PEGDA) • Photoinitiator: 1% Irgacure 819 • UV absorber: 2% 2-nitrophenyl phenyl sulfide Figure 1. 3D printed devices for affinity extraction. (A) Image of stereolithography 3D printer. (B) OpenSCAD design. Reservoirs are yellow, channels are green, the monolith polymerization window is blue, and the device divisions are red. (C) Cross section of device design. (D) Photograph of 3D printed device. IMMUNOAFFINITY EXTRACTION Monolith formation 1 • Monomer: 24% glycidyl methacrylate (GMA) • Crosslinker: 11% ethylene glycol dimethacrylate • Porogens: 10% cyclohexanol and 55% 1- dodecanol • Photoinitiator: 1% 2,2 dimethoxy-2-phenyl acetophenone Monolith is exposed to UV light for 10 minutes to polymerize. REFERENCES [1] Parker EP, Nielsen AV, Beauchamp MJ, Almughamsi HM, et al. Anal Bioanal Chem. 2019; 411: 5405-5413. [2] Gong H, Bickham BP, Woolley AT, Nordin GP. Lab chip. 2017; 17: 2899-2909 . ACKNOWLEDGEMENTS • National Institutes of Health (R01 EB027096) • BYU Department of Chemistry and Biochemistry • BYU Department of Electrical and Computer Engineering • Taif University, College of Science, Department of Chemistry