SiM20 Posters

Sensors in Medicine 2020 40 A novel commercially fabricatedmicrosystem for electrochemical detection of procalcitonin in human serum Towards a novel commercially fabricated microsystem for electrochemical detection of procalcitonin in human serum Uroš Zup nčič, Pedro Estrela, and Despina Moschou A novel commercially fabricatedmicrosystem for electrochemical detection of procalcitonin in human serum Background A novel commercially fabricatedmicrosystem for electrochemical detection of procalcitonin in human serum Sensor construction Procalcitonin quantification for sepsis detection and progression monitoring is currently performed in centralized labs or using semi-quantitative paper-based assays. 1 Here we present electrochemical quantification of procalcitonin using commercial printed circuit board (PCB) platform. Integration of this sensor in microfluidic microsystem could enable low cost, upscalable solution for sepsis diagnostics. A novel commercially fabricatedmicrosystem for electrochemical detection of procalcitonin in human serum Surface preparation PCB electrode DSP Capturing mAb BSA Blocking Sample Label mAb Streptavidin-pHRP TMB (30 min) (15 min) (5 min) (1 min) Centre for Biosensors, Bioelectronics and Biodevices (C3Bio) and Department of Electronic & Electrical Engineering, University of Bath, UK • Electrode cleaning • Incubation in DSP • C. mAb (overnight) • Quenching (TBS) • Blocking (BSA) • Sample in undiluted serum • Biotinylated mAb • Streptavidin-polyHRP • Precipitating TMB 2 • PBS (measurement) CE WE 1 WE 2 WE 3 • Cyclic voltammetry scan at 1 V/s in PBS reveals oxidation of precipitated TMB. Set-up and Readout • On board gold PCB CE and WEs were used along Ag/AgCl (KCl) reference electrode. • pTMB oxidation peak is integrated for quantification. From sample to result