SiM20 Posters

41 Sensors in Medicine 2020 A novel commercially fabricatedmicrosystem for electrochemical detection of procalcitonin in human serum Results A novel commercially fabricatedmicrosystem for electrochemical detection of procalcitonin in human serum Future directions A novel commercially fabricatedmicrosystem for electrochemical detection of procalcitonin in human serum Acknowledgements A novel commercially fabricatedmicrosystem for electrochemical detection of procalcitonin in human serum References a) Detection of PCT on PCBs b) c) d) e) Development of PCB based microsystem 500 µm a) Increasing PCT concentration in serum reveals higher pTMB oxidation peaks allowing quantification. b) Conceptual representation of microchannel integrated PCB electrodes. c) Micromixer integration into PCB based microsystem. d) Design of various features in the microsystem, from top to bottom: microchannel expansion from 0.5 mm to 1 mm channel width, ignoration of electrodes within the microchannel, U- turn part of the micromixer, two-channels joining. e) The same features manufactured by PCB standard manufacturing. • Demonstration of procalcitonin qu ntification using PCB electrodes under flow conditions in the microfluidic channel. • Integration of all electrochemical ELISA assay steps within the fully enclosed PCB based microsystem • Microsystem assay testing using whole blood. U. Zupančič is funded by University of Bath, Santander and Rosetrees Trust project M681 “Printed biosensors for next generation sepsis Point-of-Care diagnostic Lab-on-Chip microsystems”. 1. Reddy et al., Point-of-care sensors for the management of sepsis. Nature Biomedical Engineering 2018, 2 (9), 640-648. 2. Sabaté del Río et al., An antifouling coating that enables affinity-based electrochemical biosensing in complex biological fluids. Nature Nanotechnology 2019, 14 (12), 1143-1149.