SiM20 Posters

Sensors in Medicine 2020 8 CF5 GF/DVA VF2 Standard17 Fusion5 CF4 MF1 LF1 Prima40 Material Thickness(µm) Wickingrate(s4cm) Waterabs(mg cm -2 ) 954 785 785 482 63.3 28.2 23.8 67.3 99.2 93 86.2 49.9 370 367 247 192 38 29.7 35.6 44 40 39.4 25.3 -- 370 34.5 44.9 Autofluorescence High Low Medium High Medium Low Medium Medium Low MaximalS/N -FRONT 2.2 13.5 19 45 140 170 190 21 340 MaximalS/N -BACK 42 460 440 74 340 560 250 50 360 K. Arias-Alpízar 1,2 , A. Sánchez-Cano 1,2 , O. Alonso 3 , A. Diéguez 3 , E. Baldrich 1,4 1 Diagnostic Nanotools Group, CIBBIM ‐Nanomedicine, Vall d’Hebron Institut de Recerca (VHIR), Vall d’Hebron Barcelona Hospital Campus, Barcelona, Spain. 2 Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain. 3 Department of Electronis and Biomedical Engineering, University of Barcelona. 4 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain Malaria is curable when diagnosed on time, but it still caused 405,000 deaths worldwide in 2018. Most of the vulnerable population lives in remote regions with limited access to medical assistance, where current rapid diagnostic tests fail to provide the sensitive and quantitative response required to identify asymptomatic carriers, establish disease severity and monitor treatment response. Therefore, there is a need and an increasingly demand by stakeholders for testing devices for disease diagnosis near the patient (point-of-care, POC). POC testing could improve disease diagnosis and management, providing portability, automation, speed, cost, and efficiency which reduce the time needed for treatment, and facilitating treatment response monitoring. Here, we present the development of a fluorescent POC device for the fast, simple and quantitative detection of Plasmodium falciparum LDH (Pf-LDH). This POC entails an optimized single-step immunoassay, performed with Magnetic Beads (MB) and an immuno-modified signal amplifier, which is integrated in a single-use microfluidic paper-based analytical device (µPDA). Fluorescence signal is measured by a miniaturized, portable, and sensible (Internet-of Things, IoT) IoT-based point-of-care fluorescent detector. Results - Development of integrated µPDA Nine different type of papers were tested and three out of them (Standard 17, MF1 and Fusion 5) were chosen for evaluation, because of their low autofluorescence (see Fig 2a). Standard 17 was finally selected because presents the highest signal-to-noise ratios, S/N, it is easy to cut and its pore size allows MB flow. MALARIA QUANTITATIVE DIAGNOSIS USING A SINGLE-STEP MAGNETO-IMMUNOASSAY, PAPER MICROFLUIDICS AND A HAND-HELD FLUORESCENCE READER =MB-cAb = PolyHRP-dAb = PF-LDH = Fluorescent Substrate (QuantaRed) Single-step incubation: - MB-cAb - PolyHRP-dAb - Antigen (Pf-LDH) 5 min Washing Remove unbound antigen and dAb 2 1 Figure 1 . The single-step magneto-immunoassay of a simultaneous 5-min incubation of all the bio- reagents involve, perfor- med entirely in eppen- dorfs using a magnetic rack. 1. Micro fluidic paper sensor 1.1. Incubator pad 2.2. Lecture pad 2. Doble plastic layer 3. Magnet 4. Ethylvinylacetate Figure 2 a) Properties of nine types of papers and b) scheme of our microfluidic paper device. Introduction Results – Classical Approach (MBs single-step immunoassay) a) b) 1 2 4 3 1.2 1.1 Fluorescence Substrate incubation: - MB-cAb / PolyHRP-dAb / Antigen (Pf-LDH) - Fluorescent Substrate 5 min 1 Measure fluores- cence 2