Page 19 Joseph Broomfield 1,2, Melpomeni Kalofonou 1, Costanza Guilli 1, Sue M. Powell 2, Nick Moser 1, Stephen Mangar 2, Charlotte L. Bevan 2, Pantelis Georgiou 1. Benchtop detection of YAP1 and AR mRNA Handheld Lab-on-Chip detection RNA was extracted from blood plasma samples from prostate cancer patients and tested for YAP1 and AR expression with RT-pHLAMP. In each case, 12 out of 13 samples RT-pHLAMP detection agreed with results obtained by RT-qPCR. qPCR primers designed to cover the exact region of the fragment detected RT-pHLAMP additionally showed concordance with 12 out of 13 samples. First frame of the ISFET array 'Amplification curve' from the ISFET biosensor Preliminary data indicates the ISFET Lab-on-Chip can detect YAP1 mRNA in clinical samples. Sensor output rises during an amplification event when the target mRNA is present. Generation of a multiplex device for simultaneous detection of YAP1 mRNA and AR is ongoing. This works presents the foundation for simultaneous handheld detection of circulating mRNA for prostate cancer prognostics. Positive (top) and negative (below) sensor outputs The authors of this work would like to thank all members of the Bevan and Georgiou groups for their insights. This work was funded by a CRUK convergence PhD Scholarship. (1) Centre for BioInspired Technology, Electrical and Electronic Engineering, Imperial College London, SW2 7AZ. (2) Imperial Centre for translational and experimental medicine, Surgery and Cancer, Imperial College London, W12 0NN. Lacewing Handheld Device Acknowledgements
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