Sensor Technology Page 3. 4 It is also to be expected that the technology will evolve, seeking for an improved quantification and lower detection limits, integration within the signal readout as part of other techniques rather than optical, such as electrochemical measurements, or even combined approaches that can improve the performance, such as electrophoresis.4 Re garding the bioreceptors immobilized in the test (in the nanoconjugate, test and control lines), it is highly probable that LFBs will move from the immunoreactions (i.e. antibodies) to molecular sensing (i.e. DNA strains, aptamers and derivates), since DNA is more stable and affordable than antibodies. In addition, by using the DNA-based detection the integration of isothermal amplification methods (even inside the paper strip), greatly lowering the detection limit, such as Loop-Mediated Isothermal Amplification (LAMP) or Recombinase Polymerase Amplification (RPA) may be expected.5 References 1. Charlton, D. E. (1988).Test device and method for colored particle immunoassay. Patent: US6485982B1. 2.Wong, R. & Tse, H. (2009). Lateral Flow Immunoassay, Springer Link, ISBN: 978-1-59745240-3. 3. Nguyen,V.T.; Song, S.; Park, S. & Joo, C. (2020). Recent advances in high-sensitivity detection methods for paper-based lateral-flow assay. Biosens. Bioelectron., 152, 112015. 4. Sena-Torralba,A.; Álvarez-Diduk, R.; Parolo, C.; Piper,A. & Merkoçi,A. (2022).Toward Next Generation Lateral Flow Assays: Integration of Nanomaterials. Chem. Rev., 122, 18, 14881–14910. 5. Rubio-Monterde,A.; Quesada-González, D. & Merkoçi,A. (2023).Toward Integrated Molecular Lateral Flow Diagnostic Tests Using Advanced Micro- and Nanotechnology. Anal. Chem., 95, 1, 468–489. Catalan Institute of Nanoscience and Nanotechnology (ICN2)
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